Detection in vivo of a new gene product (gene III) of cauliflower mosaic virus.

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

Simultaneous Detection of Arabis Mosaic Virus, Cherry Leafroll Virus and Cucumber Mosaic Virus with Coamplification of Plant mRNA as Internal Control for Olive Certification Programs

Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...

Detection of a subgenomic mRNA for gene V, the putative reverse transcriptase gene of cauliflower mosaic virus.

Polypeptides synthesized in vitro in rabbit reticulocyte lysates, directed by poly(A)+ RNA isolated from turnip leaves infected with cauliflower mosaic virus (CaMV), were analysed by polyacrylamide gel electrophoresis. Following translation of virus-specific RNA purified by hybrid-selection using CaMV DNA immobilized on DBM papers, the polypeptides observed included the viral gene VI inclusion ...

Partial Epitope Mapping of Alfalfa mosaic Virus and the Effect of Coat Protein Gene Mutation on Aphid Transmission

The Role of Gene Therapy in Cartilage Repair

The key principle of gene delivery to articulations by direct intra-articular injection is to release complementary DNA(cDNA)-encoding medical products that will lead to maintained, endogenous production of the gene products withinthe articulation. In fact, this has been accomplished for both in vivo and ex vivo gene delivery, using several vectors,genes, and cells in some animal models. Some c...